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FAQs
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IsoFast™ Hot Start Bst Polymerase Colour & Mix FAQs
Can I use products amplified with IsoFast® Hot Start Bst reagents in downstream applications, such as cloning or sequencing?
Certain types of isothermal amplification strategies lend themselves to downstream applications, e.g. RCA (rolling circle amplification) or WGA (whole genome amplification) for NGS. IsoFast® Hot Start Bst reagents can be used for this type of amplification. However, the most common type of isothermal amplification, LAMP, generates multiple species of DNA products, making it unsuitable for cloning and impractical for NGS applications.
What type of nucleic acid can I amplify with IsoFast® Hot Start Bst reagents?
IsoFast® Hot Start Bst Polymerase, IsoFast® Hot Start Bst Mix, IsoFast® Hot Start Bst Colour Mix, and IsoFast® Hot Start Bst Polymerase Colour are all designed for amplification of DNA and cDNA targets. They are not suitable for direct amplification of RNA targets.
In order to amplify RNA targets, we recommend using our IsoFast® Bst 1-Step Mix, for direct 1-step isothermal amplification. Alternatively, we suggest a 2-step format, where RNA is converted to cDNA using one of our UltraScript® reagents, followed by amplification of cDNA with the users’ preferred IsoFast® Hot Start Bst reagent.
