PCR
The process of DNA manipulation has been in use for over half a century since the advent of restriction enzymes. However it was not until Kary Mullis demonstrated the process of the Polymerase Chain Reaction (PCR) in 1983 that the manipulation of DNA was sufficiently functional for it to change the landscape of biological research.
PCR continues to be the gold standard for DNA manipulation and is still present in the majority of biological research laboratories. Although PCR is principally unchanged, the technique has developed over time so that many different polymerases are available for use depending on the application. The functionality for these applications also continues to improve as development scientists find ways to improve the reliability, yield, specificity, speed and sensitivity of DNA Polymerases.
PCR Biosystems offer a range of polymerases to suit your needs and application. These enzymes are developed using the latest advances in polymerase and buffer technology to provide high-performance kits and reagents that scientists can rely on.
PCRBIO VeriFi™ Polymerase & Mixes
Our highest fidelity enzyme, ideal for cloning, sequencing and site-directed mutagenesis. Get better, more consistent results, even with long and difficult templates.
PCRBIO HS VeriFi™ Polymerase & Mixes
Our most versatile proofreading enzyme now with AptaLock™ hot start technology for maximised specificity in your high fidelity PCR. Ideal for multiplex reactions and room-temperature setup.
PCRBIO Taq DNA Polymerase & Mixes
PCRBIO Taq DNA Polymerase is an affordable, versatile and robust enzyme for all your everyday PCR applications including genotyping, screening and library construction.
PCRBIO HS Taq DNA Polymerase & Mixes
Whether you need a hot start enzyme for high-throughput assays, automated reaction setup or the detection of a low copy viral template, PCR Biosystems offers you a robust, industry-leading enzyme to meet your needs. PCRBIO HS Taq DNA Polymerase is available in standard and custom formats to suit the specific requirements of your COVID-19 diagnostic workflow.
PCRBIO Ultra Polymerase & Mixes
Is your template GC-rich? Or low in abundance? Does your sample contain PCR inhibitors? PCRBIO Ultra Polymerase is engineered to tackle these hard to amplify templates.
PCRBIO HiFi Polymerase
Our original Pfu-derived high fidelity enzyme, PCRBIO HiFi Polymerase brings robust and reliable performance to the world of high fidelity PCR.
PCRBIO Classic Taq
PCRBIO Classic Taq is a highly purified recombinant Taq DNA Polymerase for all your everyday PCR applications including genotyping, screening and library construction.
PCRBIO 1-Step Go RT-PCR Kit
PCRBIO 1-Step Go RT-PCR Kit is a convenient, easy-to-use kit for fast and efficient cDNA synthesis and PCR in a single tube.
PCRBIO DNA Markers
PCRBIO Ladders I-IV are room temperature stable and ready for immediate gel loading. They cover a wide range (from 50bp to 10kb) and contain loading dye for simple migration observation.
PCRBIO dNTP Mix
Ultra-pure premixed aqueous solutions of dATP, dCTP, dGTP and dTTP available at a final concentration of 10mM each or 25mM each.
