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High Fidelity PCR

Product picture of PCRBIO HiFi Polymerase

High Fidelity PCR

For some applications such as cloning and site directed mutagenesis, or when preparing a template for sequencing, the number of mutations introduced during PCR needs to be kept to a minimum.

DNA polymerases occasionally incorporate mismatched bases into the extending strand, however high fidelity PCR makes use of DNA polymerases that possess a 3’-5’ exonuclease or ‘proofreading’ activity. This activity is used to excise incorrectly incorporated nucleotides, which are then replaced with the correct nucleotides, allowing faithful replication of the target DNA of interest.

PCR Biosystems offer a range of versatile and robust high fidelity enzymes engineered for all PCR applications where greater sequence accuracy is required, including applications with challenging reaction conditions such as long-range PCR, fast PCR or the amplification of GC-rich targets.

PCRBIO VeriFi Polymerase & Mixes

Our most versatile high fidelity enzyme designed for all PCR applications where greater sequence accuracy is required, together with successful amplification of long and challenging templates.

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PCRBIO HiFi Polymerase

Our original Pfu-derived high fidelity enzyme, PCRBIO HiFi Polymerase brings robust and reliable performance to the world of high fidelity PCR.

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PCRBIO Ultra Polymerase & Mixes

Is your template GC-rich? Or low in abundance? Does your sample contain PCR inhibitors? PCRBIO Ultra Polymerase is engineered to tackle these hard to amplify templates.

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