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Genotyping

Product picture of qPCRBIO Genotyping Mix Hi-ROX

Genotyping

Genotyping is the process used to investigate the genetic variants that an individual possesses by looking at the individual’s DNA sequence. Genotype refers to the heritable information carried by all living organisms. Small changes in genotype can lead to vast differences in phenotype, the observable characteristics originating from the interaction between genotype and environmental factors.

Genotyping involves comparing the identified DNA sequence against a sequence from another sample or a reference sequence. Genotyping allows researchers to examine large structural variations in DNA to small genetic changes in DNA such as single nucleotide polymorphisms (SNPs).

Real-time PCR provides a high throughput option for genotyping using molecular probes for fast and accurate results. Our qPCRBIO Genotyping Mix and Probe Mixes can be used for reliable allelic discrimination and are compatible with all dual-labelled allele-specific probes. Another real-time based system for genotyping is a post-PCR application called High Resolution Melt (HRM). Our qPCRBIO HRM Mix produces ultra-sensitive melt profiles to allow accurate and reliable SNP genotyping.

qPCRBIO Genotyping Mix

Fast, accurate and reproducible allelic discrimination for use in TaqMan® and other dual-labelled probe-based genotyping assays.

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qPCRBIO Probe Mix

A universal probe kit designed for superior sensitivity and specificity in all probe-based real-time PCR assays.

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qPCRBIO Probe Blue Mix

Contains a non-reactive blue dye for easy sample visualisation and greater precision, particularly useful when loading small volume reactions or using white plates.

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qPCRBIO HRM Mix

Reliable, accurate and cost effective detection of genetic mutations and SNPs using High Resolution Melting (HRM) analysis.

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qPCRBIO SyGreen Mix

Fast, highly sensitive and reproducible real-time PCR with minimal optimisation required.

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qPCRBIO SyGreen Blue Mix

Our real-time blue mixes contain a non-reactive blue dye to assist you during pipetting, allowing greater visibility and precision without affecting PCR performance.

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