GenotypingGenotyping is the process used to investigate the genetic variants that an individual possesses by looking at the individual’s DNA sequence. Genotype refers to the heritable information carried by all living organisms. Small changes in genotype can lead to vast differences in phenotype, the observable characteristics originating from the interaction between genotype and environmental factors.
Genotyping involves comparing the identified DNA sequence against a sequence from another sample or a reference sequence. Genotyping allows researchers to examine large structural variations in DNA to small genetic changes in DNA such as single nucleotide polymorphisms (SNPs).
Real-time PCR provides a high throughput option for genotyping using molecular probes for fast and accurate results. Our qPCRBIO Probe Mixes can be used for reliable allelic discrimination and are compatible with all dual-labelled allele-specific probes. Another real-time based system for genotyping is a post-PCR application called High Resolution Melt (HRM). Our qPCRBIO HRM Mix produces ultra-sensitive melt profiles to allow accurate and reliable SNP genotyping.
qPCRBIO Probe Mix
qPCRBIO Probe Mix is the robust choice for all your probe-based real-time PCR needs. Our universal kit can be used to reliably detect extremely low copy number targets and quantify any DNA template including genomic, cDNA and viral sequences.
qPCRBIO Probe Blue Mix
Contains a non-reactive blue dye for easy sample visualisation and greater precision, particularly useful when loading small volume reactions or using white plates.
qPCRBIO HRM Mix
Reliable, accurate and cost effective detection of genetic mutations and SNPs using High Resolution Melting (HRM) analysis.
qPCRBIO SyGreen Mix
Fast, highly sensitive and reproducible real-time PCR with minimal optimisation required.
qPCRBIO SyGreen Blue Mix
Our real-time blue mixes contain a non-reactive blue dye to assist you during pipetting, allowing greater visibility and precision without affecting PCR performance.