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Tips and Tricks
Optimise your cDNA synthesis in 5 steps
Because good quality cDNA is key to reliable experiments, we’ve put together a set of tips to help you make the most of your reverse transcription reactions.
Whether you’re doing qPCR, cloning, or constructing cDNA libraries, follow our pointers and don’t let inefficient cDNA synthesis become the stumbling block to your research success.
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Enhance your workflow with PCRBIO HS VeriFi™ Polymerase
Challenging PCRs shouldn’t be a bottleneck to your downstream research. That’s why we’ve engineered PCRBIO HS VeriFi™ Polymerase, our high fidelity polymerase with AptaLock™ hot start technology, designed to tackle even the toughest of targets.
PCRBIO HS VeriFi™ Polymerase offers improved DNA binding and increased processivity, translating to significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates.
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Test panel for SARS-CoV-2 & winter viruses using multiplex probe 1-step RT-qPCR
RT-qPCR remains a benchmark technique for nucleic acid target detection and quantification, enabling reliable analysis of multiple samples and targets in a very short amount of time.
This application note describes a reliable and rapid multiplex assay for a winter panel comprising common winter viruses: Influenza A virus, Influenza B virus, human Respiratory Syncytial Virus A2, and the novel SARS-CoV-2 virus.