Isothermal Amplification

Isothermal Amplification with Bst Polymerase & mixes

Isothermal amplification of DNA and RNA comprises a range of methods that enable amplification at a fixed temperature, without the need for thermocycling.

How does isothermal amplification work?

Instead of denaturing DNA by using high temperatures, isothermal amplification takes advantage of DNA polymerases that have high strand displacement capabilities, for example Bst or Phi29 DNA polymerases, or recombinase-polymerase amplification (RPA) mixes. These enzymes or mixes can ‘unzip’ DNA as they synthesise complementary strands, removing the need for thermocycler equipment as the reaction is carried out at one temperature. Isothermal methods making use of strand displacing DNA polymerases include multiple displacement amplification, whole genome amplification and loop mediated isothermal amplification or LAMP and could become the next gold standard in nucleic acid detection and quantification¹.

PCR Biosystems’ IsoFast® Bst range of products is based on the large fragment of Bst DNA polymerase and offers rapid and sensitive amplification of DNA and RNA targets at a constant temperature of 65°C.

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