Isothermal amplification refers to a range of methods that rely on an alternative approach to amplification that is not limited by the requirement for thermal cycling.
Instead of denaturing DNA by using high temperatures, isothermal amplification takes advantage of DNA polymerases that have high strand displacement capabilities, for example Bst or Phi29 DNA polymerases. These enzymes have the ability to ‘unzip’ DNA as they synthesise complementary strands, removing the need for thermocycler equipment as the reaction is carried out at one temperature. Isothermal methods making use of strand displacing DNA polymerases include multiple displacement amplification, whole genome amplification and loop mediated isothermal amplification or LAMP.
PCR Biosystems’ IsoFast™ Bst range of products is based on the large fragment of Bst DNA polymerase and offers rapid and sensitive amplification of DNA and RNA targets at a constant temperature of 65°C.
IsoFast™ Bst 1-Step Mix
IsoFast™ Bst 1-Step Mix is a dual enzyme system for rapid and sensitive isothermal amplification of RNA targets in one step.
IsoFast™ Bst Polymerase
IsoFast™ Bst Polymerase offers fast amplification and strong strand displacement capabilities, making it ideal for nucleic acid amplification methods such as whole genome amplification, multiple displacement amplification and isothermal amplification.