cDNA Synthesis for Cloning
cDNA CloningReverse transcription of RNA to complementary DNA (cDNA) is the first step in many molecular biology workflows including gene expression studies using real-time PCR, gene cloning and in the creation of cDNA libraries.
A cDNA library is a collection of cloned DNA sequences that are complementary to the mRNA that was extracted from a given cell or tissue. The library represents only the transcribed sequences within a specific sample and can provide a wealth of information about the identity and functionality of specific genes. cDNA libraries also provide insights into the abundance of RNA produced in a sample because the more an mRNA is expressed, the more cDNA will be produced.
When constructing cDNA libraries it is essential that the cDNA clones are representative of the original RNA population, both in terms of their full length and relative abundance. This means the choice of reverse transcriptase used in this step is of critical importance. When cloning cDNAs and creating cDNA libraries we recommend our UltraScript and UltraScript 2.0 range of products. These highly active and thermostable enzymes are capable of synthesising more full length cDNA product, even with limited amounts of RNA, RNA expressed in low levels or containing a high degree of secondary structure.
UltraScript™ Reverse Transcriptase & Kits
UltraScript is a thermostable reverse transcriptase, producing high quality cDNA ideal for a variety of downstream applications. The enzyme enables highly sensitive and accurate reverse transcription of viral RNA sequences, making it an ideal component of RT-qPCR-based virus detection tests.
UltraScript 2.0 Reverse Transcriptase & Kits
Our high capacity, highly thermostable reverse transcriptase can be used with reaction temperatures of 55-65°C and above, designed to problem-solve the most difficult RNA samples.