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Diagnostic qPCR

Product picture of PCRBIO's Real-time range of products

Diagnostic qPCR

Real-time PCR (qPCR) is a well-established method for the detection, quantification and genotyping of DNA in diagnostic applications from food to health and agriculture. Compared to often laborious immune-diagnostic methods, real-time PCR is a faster, more robust and sensitive method.

Real-time PCR allows high throughput evaluation of samples at a low cost. As the technology continues to develop, PCR will remain at the forefront of diagnostics and will aid in the movement towards precision medicine in the future. A challenge of using real-time PCR in diagnostic applications is that the samples can require preparation so that inorganic substances present in the sample do not inhibit the PCR. All of our real-time PCR mixes are resistant to inhibitors due to our optimised buffer chemistry, meaning consistent and reliable results each time, even under difficult conditions.

Our antibody-mediated hot start technology prevents non-specific reactions and primer dimer formation at room temperature making our real-time mixes the optimal choice as a component of a diagnostic kit or in a high throughput diagnostic lab. We have strict quality control procedures meaning we provide batch to batch consistency, and we are ISO 13485:2016 certified, meaning all our products are fully traceable. We provide the flexibility to tailor our products to your diagnostic qPCR needs.

qPCRBIO Probe 1-Step Virus Detect

A high-concentration 4x RT-qPCR kit designed specifically for ultra-sensitive, high-throughput detection of viral RNA sequences, including SARS-CoV-2. Users can add more sample to their reactions to boost analytical sensitivity, even when working with small volume reactions.

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qPCRBIO Probe 1-Step Go

This universal 2x master mix is engineered for fast and sensitive 1-step RT-qPCR using sequence-specific probes. The redesigned buffer system, thermostable reverse transcriptase and antibody-mediated hot start technology support rapid and accurate detection of the SARS-CoV-2 virus.

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qPCRBIO Probe Mix

qPCRBIO Probe Mix is the robust choice for all your probe-based real-time PCR needs. Our universal kit can be used to reliably detect extremely low copy number targets and quantify any DNA template including genomic, cDNA and viral sequences.

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qPCRBIO Probe Blue Mix

Contains a non-reactive blue dye for easy sample visualisation and greater precision, particularly useful when loading small volume reactions or using white plates.

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qPCRBIO SyGreen Mix

Fast, highly sensitive and reproducible real-time PCR with minimal optimisation required.

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qPCRBIO SyGreen Blue Mix

Our real-time blue mixes contain a non-reactive blue dye to assist you during pipetting, allowing greater visibility and precision without affecting PCR performance.

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qPCRBIO SyGreen 1-Step Kits

qPCRBIO SyGreen 1-Step Kits have been designed for fast, highly specific and ultra-sensitive cDNA synthesis and real-time PCR in a single tube.

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