Diagnostic qPCRReal-time PCR (qPCR) is a well-established method for the detection, quantification and genotyping of DNA in diagnostic applications from food to health and agriculture. Compared to often laborious immune-diagnostic methods, real-time PCR is a faster, more robust and sensitive method.
Real-time PCR allows high throughput evaluation of samples at a low cost. As the technology continues to develop, PCR will remain at the forefront of diagnostics and will aid in the movement towards precision medicine in the future. A challenge of using real-time PCR in diagnostic applications is that the samples can require preparation so that inorganic substances present in the sample do not inhibit the PCR. All of our real-time PCR mixes are resistant to inhibitors due to our optimised buffer chemistry, meaning consistent and reliable results each time, even under difficult conditions.
Our antibody-mediated hot start technology prevents non-specific reactions and primer dimer formation at room temperature making our real-time mixes the optimal choice as a component of a diagnostic kit or in a high throughput diagnostic lab. We have strict quality control procedures meaning we provide batch to batch consistency, and we are ISO 13485:2016 certified, meaning all our products are fully traceable. We provide the flexibility to tailor our products to your diagnostic qPCR needs.
qPCRBIO Probe 1-Step Virus Detect
A high-concentration 4x RT-qPCR kit designed specifically for ultra-sensitive, high-throughput detection of viral RNA sequences, including SARS-CoV-2. Users can add more sample to their reactions to boost analytical sensitivity, even when working with small volume reactions.
qPCRBIO Probe 1-Step Go
This universal 2x master mix is engineered for fast and sensitive 1-step RT-qPCR using sequence-specific probes. The redesigned buffer system, thermostable reverse transcriptase and antibody-mediated hot start technology support rapid and accurate detection of the SARS-CoV-2 virus.
RiboShield™ RNase Inhibitor
RiboShield™ RNase Inhibitor is a recombinant protein that blocks the activity of a wide range of ribonucleases to reliably protect your RNA from RNase digestion in all RNA-sensitive applications.
qPCRBIO Probe Mix
qPCRBIO Probe Mix is the robust choice for all your probe-based real-time PCR needs. Our universal kit can be used to reliably detect extremely low copy number targets and quantify any DNA template including genomic, cDNA and viral sequences.
qPCRBIO Probe Blue Mix
Contains a non-reactive blue dye for easy sample visualisation and greater precision, particularly useful when loading small volume reactions or using white plates.
qPCRBIO SyGreen Mix
Fast, highly sensitive and reproducible real-time PCR with minimal optimisation required.
qPCRBIO SyGreen 1-Step Kits
qPCRBIO SyGreen 1-Step Kits have been designed for fast, highly specific and ultra-sensitive cDNA synthesis and real-time PCR in a single tube.