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Diagnostic qPCR

Product picture of PCRBIO's Real-time range of products

Diagnostic qPCR

Real-time PCR (qPCR) is a well-established method for the detection, quantification and genotyping of DNA in diagnostic applications from food to health and agriculture. Compared to often laborious immune-diagnostic methods, real-time PCR is a faster, more robust and sensitive method.

Real-time PCR allows high throughput evaluation of samples at a low cost. As the technology continues to develop, PCR will remain at the forefront of diagnostics and will aid in the movement towards precision medicine in the future. A challenge of using real-time PCR in diagnostic applications is that the samples can require preparation so that inorganic substances present in the sample do not inhibit the PCR. All of our real-time PCR mixes are resistant to inhibitors due to our optimised buffer chemistry, meaning consistent and reliable results each time, even under difficult conditions.

Our antibody-mediated hot start technology prevents non-specific reactions and primer dimer formation at room temperature making our real-time mixes the optimal choice as a component of a diagnostic kit or in a high throughput diagnostic lab. We have strict quality control procedures meaning we provide batch to batch consistency, and we are ISO 13485:2016 certified, meaning all our products are fully traceable. We provide the flexibility to tailor our products to your diagnostic qPCR needs.

qPCRBIO Probe Mix

qPCRBIO Probe Mix is the robust choice for all your probe-based real-time PCR needs. Our universal kit can be used to reliably detect extremely low copy number targets and quantify any DNA template including genomic, cDNA and viral sequences.

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qPCRBIO Probe Blue Mix

Contains a non-reactive blue dye for easy sample visualisation and greater precision, particularly useful when loading small volume reactions or using white plates.

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qPCRBIO Probe 1-Step Go

Our recommended product for scientists developing SARS-CoV-2 diagnostic tests. This universal 2x master mix is engineered for fast and sensitive 1-step RT-qPCR. The redesigned buffer system, thermostable reverse transcriptase and antibody-mediated hot start technology support rapid and accurate detection of the SARS-CoV-2 virus.

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qPCRBIO SyGreen Mix

Fast, highly sensitive and reproducible real-time PCR with minimal optimisation required.

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qPCRBIO SyGreen Blue Mix

Our real-time blue mixes contain a non-reactive blue dye to assist you during pipetting, allowing greater visibility and precision without affecting PCR performance.

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qPCRBIO SyGreen 1-Step Kits

qPCRBIO SyGreen 1-Step Kits have been designed for fast, highly specific and ultra-sensitive cDNA synthesis and real-time PCR in a single tube.

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qPCRBIO Genotyping Mix

Fast, accurate and reproducible allelic discrimination for use in TaqMan® and other dual-labelled probe-based genotyping assays.

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qPCRBIO HRM Mix

Reliable, accurate and cost effective detection of genetic mutations and SNPs using High Resolution Melting (HRM) analysis.

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