PCRBIO DNA Markers

PCRBIO Ladders I-IV are designed for easy size determination and DNA quantification using agarose gel electrophoresis. 

The ladders are room temperature stable and ready for immediate gel loading. They cover a wide range (from 50bp to 10kb) and contain loading dye for simple migration observation. 

Features

  • Ready to use – load straight onto your gel 
  • Room temperature stable – store at 25°C 
  • Quantitative – helps to visualise PCR yield 
  • Wide range – 50bp to 10kb 
  • Evenly spaced bands 
  • Easy to identify reference bands 
CAT No.
Product
Size
Price
QTY
PB40.11-01
PCRBIO Ladder I
100 Lanes
$55.00
PB40.11-05
PCRBIO Ladder I
500 Lanes
$270.00
PB40.12-01
PCRBIO Ladder II
100 Lanes
$40.00
PB40.12-05
PCRBIO Ladder II
500 Lanes
$134.00
PB40.13-01
PCRBIO Ladder III
100 Lanes
$55.00
PB40.13-05
PCRBIO Ladder III
500 Lanes
$270.00
PB40.14-01
PCRBIO Ladder IV
100 Lanes
$40.00
PB40.14-05
PCRBIO Ladder IV
500 Lanes
$134.00

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More Information

PCRBIO Ladders I-IV are manufactured using a combination of plasmid restriction digest fragments and PCR amplification products. The ladders are resuspended in sample buffer ready for immediate gel loading. The buffer formulation also allows for greater room temperature stability compared to conventional DNA ladders.

 

Applications

  • Agarose gel electrophoresis

Specifications

PCRBIO Ladder I - IV

Component

100 Lanes

500 Lanes

PCRBIO Ladder

1 x 500μL

5 x 500μL

6x Loading Dye A

1 x 400μL

1 x 2mL

PCRBIO Ladder I - IV

Component

PCRBIO Ladder

6x Loading Dye A



100 Lanes

1 x 500μL

1 x 400μL

500 Lanes

5 x 500μL

1 x 2mL

Reaction Volume

Storage

Not applicable

Store at 25°C for 6 months

Store at 4°C for 12 months

Store between -30°C and -15°C for 24 months

Reaction Volume

Not applicable

Storage

Store at 25°C for 6 months

Store at 4°C for 12 months

Store between -30°C and -15°C for 24 months

FAQs

Are PCRBIO Ladders stable at room temperature?

The ladders are already suspended in sample buffer and are ready for gel loading. The buffer formulation also allows for greater room temperature stability compared to conventional DNA ladders. Our ladders match or exceed stability of the best on the market.

What are the recommended applications for PCRBIO Ladders?

PCRBIO Ladders are designed for size determination and DNA quantification using agarose electrophoresis.

What are the sizes of PCRBIO ladders?

PCRBIO Ladder I (PB40.11): 100 – 10,000 bp

PCRBIO Ladder II (PB40.12): 250 – 10,000 bp

PCRBIO Ladder III (PB40.13): 50 – 1,500 bp

PCRBIO Ladder IV (PB40.14): 100 – 1,500 bp

Why do PCRBIO Ladders appear diffused on E-Gel Precast Agarose Electrophoresis System and how can I fix this?

The precast agarose systems are convenient however, there is a degree of compromise on the resolution. Any ladders will appear at much lower resolution on these systems. This is mainly due to the limited control they allow over the voltage as well as the gels themselves. A partial fix is to load less and ensure the ladder runs as far as it can. If the quality is still insufficient, we recommend using more traditional tanks and freshly cast gels.

More Information

PCRBIO Ladders I-IV are manufactured using a combination of plasmid restriction digest fragments and PCR amplification products. The ladders are resuspended in sample buffer ready for immediate gel loading. The buffer formulation also allows for greater room temperature stability compared to conventional DNA ladders.

 

Applications

  • Agarose gel electrophoresis

Specifications

PCRBIO Ladder I - IV

Component

100 Lanes

500 Lanes

PCRBIO Ladder

1 x 500μL

5 x 500μL

6x Loading Dye A

1 x 400μL

1 x 2mL

PCRBIO Ladder I - IV

Component

PCRBIO Ladder

6x Loading Dye A



100 Lanes

1 x 500μL

1 x 400μL

500 Lanes

5 x 500μL

1 x 2mL

Reaction Volume

Storage

Not applicable

Store at 25°C for 6 months

Store at 4°C for 12 months

Store between -30°C and -15°C for 24 months

Reaction Volume

Not applicable

Storage

Store at 25°C for 6 months

Store at 4°C for 12 months

Store between -30°C and -15°C for 24 months

FAQs

Are PCRBIO Ladders stable at room temperature?

The ladders are already suspended in sample buffer and are ready for gel loading. The buffer formulation also allows for greater room temperature stability compared to conventional DNA ladders. Our ladders match or exceed stability of the best on the market.

What are the recommended applications for PCRBIO Ladders?

PCRBIO Ladders are designed for size determination and DNA quantification using agarose electrophoresis.

What are the sizes of PCRBIO ladders?

PCRBIO Ladder I (PB40.11): 100 – 10,000 bp

PCRBIO Ladder II (PB40.12): 250 – 10,000 bp

PCRBIO Ladder III (PB40.13): 50 – 1,500 bp

PCRBIO Ladder IV (PB40.14): 100 – 1,500 bp

Why do PCRBIO Ladders appear diffused on E-Gel Precast Agarose Electrophoresis System and how can I fix this?

The precast agarose systems are convenient however, there is a degree of compromise on the resolution. Any ladders will appear at much lower resolution on these systems. This is mainly due to the limited control they allow over the voltage as well as the gels themselves. A partial fix is to load less and ensure the ladder runs as far as it can. If the quality is still insufficient, we recommend using more traditional tanks and freshly cast gels.