FAQs
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FAQs
Browse our frequently asked technical questions by selecting a product below.
What type of nucleic acid can I amplify with IsoFast® Hot Start Bst reagents?
IsoFast® Hot Start Bst Polymerase, IsoFast® Hot Start Bst Mix, IsoFast® Hot Start Bst Colour Mix, and IsoFast® Hot Start Bst Polymerase Colour are all designed for amplification of DNA and cDNA targets. They are not suitable for direct amplification of RNA targets.
In order to amplify RNA targets, we recommend using our IsoFast® Bst 1-Step Mix, for direct 1-step isothermal amplification. Alternatively, we suggest a 2-step format, where RNA is converted to cDNA using one of our UltraScript® reagents, followed by amplification of cDNA with the users’ preferred IsoFast® Hot Start Bst reagent.
