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Cloning

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Cloning

Molecular cloning is a collection of experimental methods used in molecular biology to assemble recombinant DNA molecules and to direct their replication within host organisms.

A molecular cloning reaction is typically comprised of two components which are the DNA fragment of interest to be replicated (the insert) and a vector or plasmid backbone that contains all of the components for replication in the host. The DNA fragment of interest usually has to be amplified by PCR before it can be cloned into the expression vector. There are several different methods used for cloning PCR products and PCR Biosystems offers a wide range of DNA polymerases to best suit your cloning experiment, including standard PCR (both 3’A and blunt end), high fidelity PCR and speciality PCR, such as the amplification of long amplicons or GC-rich targets.

PCRBIO VeriFi Polymerase & Mixes

Our most versatile high fidelity enzyme designed for all PCR applications where greater sequence accuracy is required, together with successful amplification of long and challenging templates.

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PCRBIO HiFi Polymerase

Our original Pfu-derived high fidelity enzyme, PCRBIO HiFi Polymerase brings robust and reliable performance to the world of high fidelity PCR.

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PCRBIO Ultra Polymerase & Mixes

Is your template GC-rich? Or low in abundance? Does your sample contain PCR inhibitors? PCRBIO Ultra Polymerase is engineered to tackle these hard to amplify templates.

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PCRBIO HS Taq DNA Polymerase & Mixes

Whether you need a hot start assay for high-throughput, automated reaction setup or the detection of a low copy number template, PCR Biosystems offers you a robust industry-leading enzyme to meet your needs.

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PCRBIO Taq DNA Polymerase & Mixes

PCRBIO Taq DNA Polymerase is an affordable, versatile and robust enzyme for all your everyday PCR applications including genotyping, screening and library construction.

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PCRBIO Classic Taq

PCRBIO Classic Taq is a highly purified recombinant Taq DNA Polymerase for all your everyday PCR applications including genotyping, screening and library construction.

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PCRBIO 1-Step Go RT-PCR Kit

PCRBIO 1-Step Go RT-PCR Kit is a convenient, easy-to-use kit for fast and efficient cDNA synthesis and PCR in a single tube.

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