GC-Rich PCRDNA templates with high GC content (>65%) can affect the efficiency of PCR due to the tendency of these templates to fold into complex secondary structures.
This is due to increased hydrogen bonding between guanine and cytosine bases, which can cause the DNA to be resistant to melting. The Taq DNA polymerase can stall at these secondary structures and primer annealing may be hindered, resulting in incomplete or non-specific amplification.
When working with GC-rich templates, we recommend PCRBIO HS Taq DNA Polymerase and PCRBIO Ultra Polymerase which are designed to perform in demanding applications. Our advanced buffer and antibody-mediated hot start technology allows robust performance and high sensitivity across a wide range templates, including complex genomic DNA and targets with a high GC content (up to 80% GC). When higher levels of sequence accuracy are required, PCRBIO HiFi Polymerase and PCRBIO VeriFi Polymerase also offer increased PCR success rates with these challenging templates.
PCRBIO HS Taq DNA Polymerase & Mixes
Whether you need a hot start assay for high-throughput, automated reaction setup or the detection of a low copy number template, PCR Biosystems offers you a robust industry-leading enzyme to meet your needs.
PCRBIO Ultra Polymerase & Mixes
Is your template GC-rich? Or low in abundance? Does your sample contain PCR inhibitors? PCRBIO Ultra Polymerase is engineered to tackle these hard to amplify templates.
PCRBIO VeriFi Polymerase & Mixes
Our most versatile high fidelity enzyme designed for all PCR applications where greater sequence accuracy is required, together with successful amplification of long and challenging templates.
PCRBIO HiFi Polymerase
Our original Pfu-derived high fidelity enzyme, PCRBIO HiFi Polymerase brings robust and reliable performance to the world of high fidelity PCR.