GC-Rich PCRDNA templates with high GC content (>65%) can affect the efficiency of PCR due to the tendency of these templates to fold into complex secondary structures.
This is due to increased hydrogen bonding between guanine and cytosine bases, which can cause the DNA to be resistant to melting. The Taq DNA polymerase can stall at these secondary structures and primer annealing may be hindered, resulting in incomplete or non-specific amplification.
When working with GC-rich templates, we recommend PCRBIO HS Taq DNA Polymerase and PCRBIO Ultra Polymerase which are designed to perform in demanding applications. Our advanced buffer and antibody-mediated hot start technology allows robust performance and high sensitivity across a wide range templates, including complex genomic DNA and targets with a high GC content (up to 80% GC).
When higher levels of sequence accuracy are required, the VeriFi™ family of high fidelity enzymes also offer increased PCR success rates with these challenging templates. The ability to withstand high temperature cycling and denaturation up to 100°C allow better separation of GC-rich sequences, making more of your template available for priming.
PCRBIO HS Taq DNA Polymerase & Mixes
Whether you need a hot start enzyme for high-throughput assays, automated reaction setup or the detection of a low copy viral template, PCRBIO HS Taq DNA Polymerase is a robust, industry-leading enzyme to meet your needs. Available in standard and custom formats to suit the specific requirements of your COVID-19 diagnostic workflow.
PCRBIO Ultra Polymerase & Mixes
Is your template GC-rich? Or low in abundance? Does your sample contain PCR inhibitors? PCRBIO Ultra Polymerase is engineered to tackle these hard to amplify templates.
VeriFi™ Hot Start Polymerase & Mixes
Our most versatile proofreading enzyme now with AptaLock™ hot start technology for maximised specificity in your high fidelity PCR. Ideal for multiplex reactions and room-temperature setup.
VeriFi™ Polymerase & Mixes
Our highest fidelity enzyme, ideal for cloning, sequencing and site-directed mutagenesis. Get better, more consistent results, even with long and difficult templates.
PCRBIO HiFi Polymerase
Our original Pfu-derived high fidelity enzyme, PCRBIO HiFi Polymerase brings robust and reliable performance to the world of high fidelity PCR.