Real-time PCR, or qPCR, is a fast developing field with wide ranging applications for researchers. From gene expression analysis and population genotyping studies, to the multiplex detection of several disease targets, real-time PCR has opened the door to a world of possibilities.
Real-time analysis is broadly divided into two areas; probe and intercalating dye-based assays. The two techniques have many overlapping applications and for the majority we recommend the use of intercalating dye technology rather than more costly dual labelled probes. Using an intercalating dye, such as SyGreen, rather than a hydrolysis probe (such as TaqMan®) has the added benefit of post amplification melt profile analysis. It also has the advantage of faster cycling than with hydrolysis probes, due to the slower 5′ to 3′ exonuclease activity of Taq DNA polymerase compared to its polymerisation rate.
The principle advantage of probe-based technologies is that multiple amplicons can be detected in the same tube. The number is typically limited by the number of light channels found in the real-time PCR instrument. PCR Biosystems’ universal probe mixes are suitable for use on a wide range of probe technologies and are designed not to lose efficiency during multiplexing.
qPCRBIO Probe 1-Step Go
Our recommended product for scientists developing SARS-CoV-2 diagnostic tests. This universal 2x master mix is engineered for fast and sensitive 1-step RT-qPCR. The redesigned buffer system, thermostable reverse transcriptase and antibody-mediated hot start technology support rapid and accurate detection of the SARS-CoV-2 virus.
qPCRBIO SyGreen Mix
Fast, highly sensitive and reproducible real-time PCR with minimal optimisation required.
qPCRBIO SyGreen Blue Mix
Our real-time blue mixes contain a non-reactive blue dye to assist you during pipetting, allowing greater visibility and precision without affecting PCR performance.
qPCRBIO Probe Mix
qPCRBIO Probe Mix is the robust choice for all your probe-based real-time PCR needs. Our universal kit can be used to reliably detect extremely low copy number targets and quantify any DNA template including genomic, cDNA and viral sequences.
qPCRBIO Probe Blue Mix
Contains a non-reactive blue dye for easy sample visualisation and greater precision, particularly useful when loading small volume reactions or using white plates.
qPCRBIO HRM Mix
Reliable, accurate and cost effective detection of genetic mutations and SNPs using High Resolution Melting (HRM) analysis.
qPCRBIO Genotyping Mix
Fast, accurate and reproducible allelic discrimination for use in TaqMan® and other dual-labelled probe-based genotyping assays.
qPCRBIO SyGreen 1-Step Kits
qPCRBIO SyGreen 1-Step Kits have been designed for fast, highly specific and ultra-sensitive cDNA synthesis and real-time PCR in a single tube.