qPCR reagents for any detection method
What is qPCR?
Real-time PCR, or quantitative PCR (qPCR), is one of the most reliable detection and quantification methods for specific DNA and RNA molecules. It is a fast-developing field with wide ranging applications for researchers. Find top-quality qPCR reagents for any application, from gene expression analysis, and population genotyping studies, to the multiplex detection of several disease targets simultaneously. Real-time qPCR is one of the most powerful tools at your disposal.
qPCR detection methods
Real-time qPCR is broadly divided into two categories based on the method of signal generation: probe-based qPCR and intercalating dye-based qPCR. These two qPCR techniques have many overlapping applications and for the majority we recommend the use of intercalating dye technology rather than more costly dual labelled probes. Using an intercalating dye, such as SyGreen, rather than a hydrolysis probe (such as TaqMan®) has the added benefit of post amplification melt profile analysis. It also has the advantage of faster cycling than with hydrolysis probes, due to the slower 5′ to 3′ exonuclease activity of Taq DNA polymerase compared to its polymerisation rate.
The main advantage of probe-based detection is that multiple amplicons can be detected in the same tube in a multiplex qPCR reaction. The number of targets is typically limited by the number of light channels found in the real-time PCR instrument. PCR Biosystems’ universal probe mixes are suitable for use with a wide range of probe technologies, across all real time thermocyclers, and are designed not to lose efficiency during multiplexing.
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Find our qPCR reagents and kits below
qPCRBIO Probe 1-Step Virus Detect
A high-concentration 4x RT-qPCR kit designed specifically for ultra-sensitive, high-throughput detection of viral RNA sequences, including SARS-CoV-2. Users can add more sample to their reactions to boost analytical sensitivity, even when working with small volume reactions.
qPCRBIO Probe 1-Step Go
This universal 2x master mix is engineered for fast and sensitive 1-step RT-qPCR using sequence-specific probes. The redesigned buffer system, thermostable reverse transcriptase and antibody-mediated hot start technology support rapid and accurate detection of the SARS-CoV-2 virus.
qPCRBIO SyGreen Mix
Fast, highly sensitive and reproducible real-time PCR with minimal optimisation required.
qPCRBIO SyGreen Blue Mix
Our real-time blue mixes contain a non-reactive blue dye to assist you during pipetting, allowing greater visibility and precision without affecting PCR performance.
qPCRBIO Probe Mix
qPCRBIO Probe Mix is the robust choice for all your probe-based real-time PCR needs. Our universal kit can be used to reliably detect extremely low copy number targets and quantify any DNA template including genomic, cDNA and viral sequences.
qPCRBIO Probe Blue Mix
Contains a non-reactive blue dye for easy sample visualisation and greater precision, particularly useful when loading small volume reactions or using white plates.
qPCRBIO HRM Mix
Reliable, accurate and cost effective detection of genetic mutations and SNPs using High Resolution Melting (HRM) analysis.
qPCRBIO SyGreen 1-Step Kits
qPCRBIO SyGreen 1-Step Kits have been designed for fast, highly specific and ultra-sensitive cDNA synthesis and real-time PCR in a single tube.