FAQs
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FAQs
Browse our frequently asked technical questions by selecting a product below.
Can I use products amplified with IsoFast® Hot Start Bst reagents in downstream applications, such as cloning or sequencing?
Certain types of isothermal amplification strategies lend themselves to downstream applications, e.g. RCA (rolling circle amplification) or WGA (whole genome amplification) for NGS. IsoFast® Hot Start Bst reagents can be used for this type of amplification. However, the most common type of isothermal amplification, LAMP, generates multiple species of DNA products, making it unsuitable for cloning and impractical for NGS applications.
