Isothermal Amplification

Isothermal Amplification

Isothermal amplification encompasses nucleic acid amplification methods that take place at a single temperature and hence do not require thermal cycling.

Such approaches utilise DNA polymerases with strong strand displacement capabilities, which enable the separation of complimentary DNA template strands during polymerisation, without the need for a separate denaturation step. This allows target amplification at a single incubation temperature. An increasing number of applications exploit this powerful combination of enzyme activities to facilitate target amplification under a wide range of conditions, making them ideal for point-of-care diagnostics, field-based detection, and fast diagnostic methods.

PCR Biosystems’ IsoFast® Bst range of products rely on the large fragment of Bst DNA polymerase and offers rapid and sensitive amplification of DNA and RNA targets at a wide range of temperatures.

IsoFast Hot Start Archive Image

IsoFast® Hot Start Bst Polymerase & Mixes

IsoFast™ Hot Start Bst Polymerase and Mixes offer cutting edge performance in all isothermal amplification workflows, LAMP applications and POC testing.

View products
IsoFast Hot Start Colour Category

IsoFast® Hot Start Bst Polymerase Colour & Mix

IsoFast® Hot Start Colour Mix allows isothermal amplification with colourimetric readout for rapid point-of-care testing and positive/negative screening.

View products

IsoFast® Bst 1-Step Mix

IsoFast® Bst 1-Step Mix is a dual enzyme system for rapid and sensitive isothermal amplification of RNA targets in one step.

View products

IsoFast® Bst Polymerase

IsoFast® Bst Polymerase offers fast amplification and strong strand displacement capabilities, making it ideal for nucleic acid amplification methods such as whole genome amplification, multiple displacement amplification and isothermal amplification.

View products