Crude Sample PCR

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Crude Sample PCR

Performing PCR direct from crude samples can be highly beneficial due to greatly reduced experimental time, cost and sample loss, particularly for high volume genotyping, transgene detection, knockout analysis or PCR-based clinical and forensic tests. A major problem however can be the presence of inhibitors to PCR which may lead to low sensitivity reactions, low yields or false positives.

PCRBIO HS Taq DNA Polymerase and PCRBIO Ultra Polymerase are designed to perform in demanding applications with high efficiency and improved tolerance to common inhibitors. Our advanced buffer and antibody-mediated hot start technology allows robust performance and high sensitivity across a wide range templates, including GC and AT-rich, together with easy reaction setup at room temperature. Furthermore, the convenient ready mix format, including the option of a red dye for direct gel loading, increases the speed and ease-of-use for successful PCR from crude samples.

For rapid DNA extraction combined with fast, highly specific DNA amplification, our PCRBIO Rapid Extract PCR Kit can be used with a range of sample types including mouse tail, buccal swab, hair follicle and blood.

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PCRBIO HS Taq DNA Polymerase & Mixes

Whether you need a hot start enzyme for high-throughput assays, automated reaction setup or the detection of a low copy viral template, PCRBIO HS Taq DNA Polymerase is a robust, industry-leading enzyme to meet your needs. Available in standard and custom formats to suit the specific requirements of your COVID-19 diagnostic workflow.

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PCRBIO Ultra Polymerase & Mixes

Is your template GC-rich? Or low in abundance? Does your sample contain PCR inhibitors? PCRBIO Ultra Polymerase is engineered to tackle these hard to amplify templates.

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PCRBIO Rapid Extract PCR Kit

Combines rapid DNA extraction with fast, highly specific DNA amplification in a convenient, easy-to-use format. Get single-tube DNA extraction without the need for spin columns or hazardous chemicals.

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