Colony PCRMolecular cloning techniques require some method of screening colonies for the presence or absence of the DNA insert. Traditionally this has been carried out with restriction enzyme digest, however colony PCR is a rapid, convenient and high throughput method for verifying that the desired genetic construct is present.
Setting up colony PCR reactions is almost identical to a standard PCR reaction however the plasmid DNA must be released from the yeast or bacteria in order to serve as the PCR template. This can either be performed by lysing in water with a short heating step or adding individual transformants directly to the PCR reaction and lysing during the initial heating step. For successful colony PCR, a robust polymerase is needed as the presence of bacterial cell contents and culture media can often result in polymerase inhibition.
PCRBIO HS Taq DNA Polymerase and PCRBIO Ultra Polymerase are designed to perform in demanding applications with high efficiency and improved tolerance to common inhibitors. Our advanced buffer and antibody-mediated hot start technology allows robust performance and high sensitivity across a wide range of templates, including GC and AT-rich, together with easy reaction setup at room temperature. Furthermore, the convenient ready mix format, including the option of a red dye for direct gel loading, increases the speed and ease-of-use for successful colony PCR experiments.
PCRBIO HS Taq DNA Polymerase & Mixes
Whether you need a hot start assay for high-throughput, automated reaction setup or the detection of a low copy number template, PCR Biosystems offers you a robust industry-leading enzyme to meet your needs.
PCRBIO Ultra Polymerase & Mixes
Is your template GC-rich? Or low in abundance? Does your sample contain PCR inhibitors? PCRBIO Ultra Polymerase is engineered to tackle these hard to amplify templates.