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Long Range PCR

Product picture of PCRBIO Ultra Mix Red

Long Range PCR

Long range PCR refers to the amplification of DNA targets over 5kb in length which typically cannot be amplified using routine PCR methods or reagents.

Traditionally, long range PCR has been performed using a blend of Taq DNA polymerase (for fast elongation) combined with a small amount of proofreading polymerase (for accuracy). The proofreading enzyme repairs DNA mismatches incorporated at the 3’ end of the growing strand, allowing Taq polymerase to continue to elongate the DNA much further than it would otherwise, resulting in longer DNA amplification.

Long range PCR can also be achieved by using modified high fidelity polymerases with enhanced DNA binding, resulting in highly processive and accurate amplification of long fragments. Whether for cloning, genome mapping or sequencing, PCR Biosystems has a range of solutions enabling the amplification of long fragments with speed and high fidelity.

PCRBIO VeriFi Polymerase & Mixes

Our most versatile high fidelity enzyme designed for all PCR applications where greater sequence accuracy is required, together with successful amplification of long and challenging templates.

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PCRBIO Ultra Polymerase & Mixes

Is your template GC-rich? Or low in abundance? Does your sample contain PCR inhibitors? PCRBIO Ultra Polymerase is engineered to tackle these hard to amplify templates.

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