PCR
PCR
The process of DNA manipulation has been in use for over half a century since the advent of restriction enzymes. However it was not until Kary Mullis demonstrated the process of the Polymerase Chain Reaction (PCR) in 1983 that the manipulation of DNA was sufficiently functional for it to change the landscape of biological research.
PCR continues to be the gold standard for DNA manipulation and is still present in the majority of biological research laboratories. Although PCR is principally unchanged, the technique has developed over time so that many different polymerases are available for use depending on the application. The functionality for these applications also continues to improve as development scientists find ways to improve the reliability, yield, specificity, speed and sensitivity of DNA Polymerases.
PCR Biosystems offer a range of polymerases to suit your needs and application. These enzymes are developed using the latest advances in polymerase and buffer technology to provide high-performance kits and reagents that scientists can rely on.
Colony PCR
Molecular cloning techniques require some method of screening colonies for the presence or absence of the DNA insert. Traditionally this has been carried out with restriction enzyme digest, however colony PCR…
Fast PCR
Enzymes capable of fast PCR can help in achieving faster turnaround times from sample to result and give improved throughput on existing thermocyclers. PCR Biosystems’ enzymes are engineered to have…
Methylated DNA
DNA methylation is a heritable epigenetic mark involving the addition of a methyl group to the 5' position of cytosine by DNA methyltransferases. It plays an important role in normal…
Cloning
Molecular cloning is a collection of experimental methods used in molecular biology to assemble recombinant DNA molecules and to direct their replication within host organisms. A molecular cloning reaction is typically comprised of two components which…
TA Cloning
TA cloning is a simple and efficient method for the cloning of PCR products. The procedure takes advantage of the terminal transferase activity of some DNA polymerases such as Taq polymerase.…
Mouse Genotyping
Mice serve as useful models in biochemical research and can be used to study the roles of genes in development, physiology and human disease. The use of these animals requires accurate…
Site Directed Mutagenesis
Site directed mutagenesis is an in vitro method for introducing specific and intentional mutations into DNA fragments. It is often performed using PCR-based methods due to its speed, simplicity and high efficiency.…
High Fidelity PCR
For some applications such as cloning and site directed mutagenesis, or when preparing a template for sequencing, the number of mutations introduced during PCR needs to be kept to a…
Crude Sample PCR
Performing PCR direct from crude samples can be highly beneficial due to greatly reduced experimental time, cost and sample loss, particularly for high volume genotyping, transgene detection, knockout analysis or…
Multiplex PCR
Multiplex PCR is a widespread technique for the amplification of multiple targets in a single PCR experiment. Multiplexing can save precious time, reagents and samples and also has the benefit…
Long Range PCR
Long range PCR refers to the amplification of DNA targets over 5kb in length which typically cannot be amplified using routine PCR methods or reagents. Traditionally, long range PCR has…
GC-Rich PCR
DNA templates with high GC content (>65%) can affect the efficiency of PCR due to the tendency of these templates to fold into complex secondary structures. This is due to…
Direct Gel Loading
Many of PCR Biosystems’ enzymes are provided in a ready-mix format, with optimised concentrations of dNTPs, MgCl2 and enhancers, requiring only the addition of template, primers and water. Our red mixes…
