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Absolute Quantification

Product picture of qPCRBIO SyGreen Mix range of pack sizes

Absolute Quantification

The most common qPCR technique, absolute quantification is an accurate way to determine the precise copy number of a gene within a sample.

The user makes a standard curve based on samples of known concentration and calculates the concentrations of unknown samples using the Cq. Using PCR Biosystems’ qPCR reagents, you will see consistent results and accurate quantification of any DNA template thanks to our 12 step purification process and thorough quality assurance steps.

qPCRBIO SyGreen Mix

Fast, highly sensitive and reproducible real-time PCR with minimal optimisation required.

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qPCRBIO SyGreen Blue Mix

Our real-time blue mixes contain a non-reactive blue dye to assist you during pipetting, allowing greater visibility and precision without affecting PCR performance.

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qPCRBIO Probe Mix

qPCRBIO Probe Mix is the robust choice for all your probe-based real-time PCR needs. Our universal kit can be used to reliably detect extremely low copy number targets and quantify any DNA template including genomic, cDNA and viral sequences.

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qPCRBIO Probe Blue Mix

Contains a non-reactive blue dye for easy sample visualisation and greater precision, particularly useful when loading small volume reactions or using white plates.

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qPCRBIO SyGreen 1-Step Kits

qPCRBIO SyGreen 1-Step Kits have been designed for fast, highly specific and ultra-sensitive cDNA synthesis and real-time PCR in a single tube.

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qPCRBIO Probe 1-Step Go

This universal 2x master mix is engineered for fast and sensitive 1-step RT-qPCR using sequence-specific probes. The redesigned buffer system, thermostable reverse transcriptase and antibody-mediated hot start technology support rapid and accurate detection of the SARS-CoV-2 virus.

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