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Absolute Quantification

Product picture of qPCRBIO SyGreen Mix range of pack sizes

Absolute Quantification

Absolute quantification by qPCR offers an accurate way to determine the precise copy number of a target nucleic acid molecule within a sample.

This approach enables the accurate quantification of a target molecule’s copy number within samples. By conducting qPCR on a dilution series of a sample with a known amount of the desired target molecule, users can generate a standard curve of Ct values corresponding to a given amount of the target. The Ct values of the samples under examination are then compared to this reference curve and a precise estimate of the target molecule’s abundance in each unknown sample calculated. This is the method of choice for many diagnostic applications and for analysis of species, allele, and transcript splice-variant abundance in a wide range of biological samples.

Regardless of the application, our qPCR reagents are engineered to help you achieve reproducible, accurate quantification. We offer market-leading sensitivity, whether you prefer dye- or probe-based detection, thanks to our proprietary hot start polymerases, robust master mixes, and visualisation dyes that help minimize pipetting errors. If your focus is diagnostic qPCR our ISO 13486:2016 certified quality management system ensures full traceability of every reagent and kit that we provide.

qPCRBIO SyGreen Mix

Fast, highly sensitive and reproducible real-time PCR with minimal optimisation required.

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qPCRBIO SyGreen Blue Mix

Our real-time blue mixes contain a non-reactive blue dye to assist you during pipetting, allowing greater visibility and precision without affecting PCR performance.

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qPCRBIO Probe Mix

qPCRBIO Probe Mix is the robust choice for all your probe-based real-time PCR needs. Our universal kit can be used to reliably detect extremely low copy number targets and quantify any DNA template including genomic, cDNA and viral sequences.

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qPCRBIO Probe Blue Mix

Contains a non-reactive blue dye for easy sample visualisation and greater precision, particularly useful when loading small volume reactions or using white plates.

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qPCRBIO SyGreen 1-Step Kits

qPCRBIO SyGreen 1-Step Kits have been designed for fast, highly specific and ultra-sensitive cDNA synthesis and real-time PCR in a single tube.

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qPCRBIO Probe 1-Step Go

This universal 2x master mix is engineered for fast and sensitive 1-step RT-qPCR using sequence-specific probes. The redesigned buffer system, thermostable reverse transcriptase and antibody-mediated hot start technology support rapid and accurate detection of the SARS-CoV-2 virus.

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