Image showing the NGSBIO Library Quant Kit for Illumina product range

NGS library amplification & quantification reagents

What is NGS?

Next generation sequencing or NGS refers to the high-throughput, in-parallel DNA and RNA sequencing technologies that became available at the onset of the 21st century.

These technologies have immense advantages in terms of cost-effectiveness, sequencing speed, resolution, and accuracy compared to the traditional Sanger first-generation sequencing method developed in the late 1970’s.

NGS is now the method of choice for large-scale genomic and transcriptomic sequencing, and metagenomic analyses. To date, these high-throughput sequencing technologies have been comprehensively applied in a variety of ways, such as whole genome sequencing, target sequencing, gene expression profiling (RNA-Seq) and small RNA sequencing, to vastly accelerate biological and biomedical research.

NGS Reagents from PCR Biosystems

VeriFi™ Library Amplification Mix is a cutting edge, proofreading Pfu polymerase engineered for reduced GC-bias and improved multiplex capability. The mix is designed for use in all types of NGS sequencing applications that require PCR-based library amplification and has been extensively validated for use with Illumina® sequencing primers and platforms.

We also supply NGSBIO Library Quant Kits. These kits use the latest developments in qPCR technology to provide accurate and rapid quantification of NGS sequencing libraries prior to sequencing. NGSBIO products are specifically engineered to support and enhance NGS workflows using Illumina® systems.

Need more help with finding the right NGS reagents and resources?

If you’re unsure about which detection method and reagents are suitable for your application take a look at our NGS applications page, or get in touch with a member of our team for a recommendation. Already using our NGSBIO Library Quantification Kits? Use our NGSBIO Library Quantification Tool. You can also request free NGS reagent samples if you wish to test these under your specific experimental conditions.

VeriFi™ Library Amplification Mix

2x PCR ready mix for NGS library amplification. Reduced GC bias & more unique reads than competitor mixes. Ideal for Illumina® sequencing platforms.

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NGSBIO Library Quant Kit Blue for Illumina®

Accurately and rapidly quantify your library prior to sequencing with Illumina® NGS systems using our blue, easy-to-see qPCR-based quantification kit enabling greater pipetting precision.

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NGSBIO Library Quant Kit for Illumina®

Accurately and rapidly quantify your library prior to sequencing with Illumina® NGS systems using our qPCR-based quantification kit.

View products