Relative Gene Expression Analysis
Relative Gene Expression AnalysisRelative quantification by PCR is a valuable tool for measuring gene expression levels. This approach relies on the comparative Cq method and gives a relative value of target molecule abundance.
Relative quantification is the method of choice to study the abundance of one or more targets in parallel. Because this analysis requires fewer control samples for quantification compared to absolute quantification methods, it is optimal for increased throughput. This type of analysis can be carried out either using intercalating dye- or probe-based detection, with different advantages and disadvantages for each approach and is ideal for gene expression studies.
At PCR Biosystems we carefully engineer qPCR reagents for both types of detection method and offer a range of master mixes designed to maximise your research output, ensuring reliable results and sensitive detection in every application.
qPCRBIO SyGreen 1-Step Kits
qPCRBIO SyGreen 1-Step Kits have been designed for fast, highly specific and ultra-sensitive cDNA synthesis and real-time PCR in a single tube.
qPCRBIO SyGreen Mix
Fast, highly sensitive and reproducible real-time PCR with minimal optimisation required.
qPCRBIO SyGreen Blue Mix
Our real-time blue mixes contain a non-reactive blue dye to assist you during pipetting, allowing greater visibility and precision without affecting PCR performance.
qPCRBIO Probe Mix
qPCRBIO Probe Mix is the robust choice for all your probe-based real-time PCR needs. Our universal kit can be used to reliably detect extremely low copy number targets and quantify any DNA template including genomic, cDNA and viral sequences.
qPCRBIO Probe Blue Mix
Contains a non-reactive blue dye for easy sample visualisation and greater precision, particularly useful when loading small volume reactions or using white plates.
qPCRBIO Probe 1-Step Go
This universal 2x master mix is engineered for fast and sensitive 1-step RT-qPCR using sequence-specific probes. The redesigned buffer system, thermostable reverse transcriptase and antibody-mediated hot start technology support rapid and accurate detection of RNA targets and viruses.
Clara™ Probe 1-Step Mix
A high-concentration 4x 1-step RT-qPCR kit in a single tube format. Enables ultra-sensitive, high-throughput detection of RNA and DNA sequences with minimal effort.
Clara™ Probe Mix
A powerful qPCR mix for probe-based detection of DNA. Compatible with all real-time qPCR instruments and probe chemistries.