Relative Gene Expression Analysis
Relative Gene Expression AnalysisRelative gene expression analysis is a valuable method of quantifying gene expression by comparing target transcripts and reference genes. Gene expression calculations can be performed using either the comparative CT or the standard curve methods.
Relative gene expression reduces the likelihood of pipetting errors and allows for increased throughput by calculating gene expression of the target and control within the same tube. To ensure accurate calculations, the amplification efficiency of target and control should be the same. Using PCR Biosystems’ qPCR reagents, you will get reliable results with early Ct values due to our enzymes’ rapid extension rate.
qPCRBIO SyGreen Mix
Fast, highly sensitive and reproducible real-time PCR with minimal optimisation required.
qPCRBIO SyGreen Blue Mix
Our real-time blue mixes contain a non-reactive blue dye to assist you during pipetting, allowing greater visibility and precision without affecting PCR performance.
qPCRBIO Probe Mix
qPCRBIO Probe Mix is the robust choice for all your probe-based real-time PCR needs. Our universal kit can be used to reliably detect extremely low copy number targets and quantify any DNA template including genomic, cDNA and viral sequences.
qPCRBIO Probe Blue Mix
Contains a non-reactive blue dye for easy sample visualisation and greater precision, particularly useful when loading small volume reactions or using white plates.
qPCRBIO Probe 1-Step Go
Our recommended product for scientists developing SARS-CoV-2 diagnostic tests. This universal 2x master mix is engineered for fast and sensitive 1-step RT-qPCR. The redesigned buffer system, thermostable reverse transcriptase and antibody-mediated hot start technology support rapid and accurate detection of the SARS-CoV-2 virus.
qPCRBIO SyGreen 1-Step Kits
qPCRBIO SyGreen 1-Step Kits have been designed for fast, highly specific and ultra-sensitive cDNA synthesis and real-time PCR in a single tube.