Real-time PCR, or qPCR, is a fast-developing field with wide ranging applications. Whatever your research goal is, from gene expression analysis, population genotyping, SNP and disease marker detection, qPCR is your go-to technique.
What is qPCR?
Real time qPCR is a type of PCR that enables both relative or absolute quantification of specific DNA and RNA targets. Detection is achieved using tagged oligonucleotide probes (such as: TaqMan®, Molecular Beacons, and Scorpions®) or DNA intercalating dyes, like SyGreen, or SYBR™ Green (depending on the manufacturer). Multiple research applications based on qPCR have been developed. These include gene expression analysis, genotyping, allelic discrimination, single nucleotide polymorphism (SNP) detection, microbe and pathogen detection, species abundance quantification and many others.
We provide a range of high quality qPCR and RT-qPCR mastermixes for 1-step and 2-step workflows suitable that can be used in any of the applications above. All of our probe-based qPCR mixes are suitable for singleplex and multiplex target detection. Additionally, we have developed optimised mastermixes for specialist qPCR applications with an emphasis on molecular diagnostics and IVD kit development. These include mastermixes for: high resolution melt curve (HRM) analysis, lyophilisable qPCR and RT-qPCR mixes, and a powerful complete air-dryable mastermix.
Find your qPCR application below
Relative Gene Expression Analysis
Relative quantification by PCR is a valuable tool for measuring gene expression levels. This approach relies on the comparative Cq method and gives a relative value of target molecule abundance.…
Absolute quantification by qPCR offers an accurate way to determine the precise copy number of a target nucleic acid molecule within a sample. This approach enables the accurate quantification of…
Real-time PCR (qPCR) is a well-established method for the detection, quantification and genotyping of DNA in diagnostic applications from food to health and agriculture. Compared to often laborious immune-diagnostic methods,…
Genotyping is the process used to investigate the genetic variants that an individual possesses by looking at the individual’s DNA sequence. Genotype refers to the heritable information carried by all…
Multiplexing is a powerful and efficient way to calculate gene expression levels of multiple targets in a single tube. Each target gene is amplified using specific primers and labelled probes…