TA CloningTA cloning is a simple and efficient method for the cloning of PCR products. The procedure takes advantage of the terminal transferase activity of some DNA polymerases such as Taq polymerase.
During amplification, this enzyme adds a single 3’-A nucleotide to the end of each PCR product. The PCR product can be easily ligated into a vector that has been cut and engineered to contain single T residues on each strand. This technique is particularly useful when compatible restriction sites are not available for the subcloning of DNA fragments from one vector to another. PCRBIO has a range of enzymes suitable for TA cloning which are listed below.
DNA polymerases with proofreading activity, such as our Pfu-derived high fidelity polymerases, can not be used for TA cloning because they generate blunt-ended PCR products. These high-fidelity enzymes can instead be used for instances where ligation of blunt-ended DNA fragments is required.
PCRBIO Taq DNA Polymerase & Mixes
PCRBIO Taq DNA Polymerase is an affordable, versatile and robust enzyme for all your everyday PCR applications including genotyping, screening and library construction.
PCRBIO HS Taq DNA Polymerase & Mixes
Whether you need a hot start assay for high-throughput, automated reaction setup or the detection of a low copy number template, PCR Biosystems offers you a robust industry-leading enzyme to meet your needs.
PCRBIO Classic Taq
PCRBIO Classic Taq is a highly purified recombinant Taq DNA Polymerase for all your everyday PCR applications including genotyping, screening and library construction.