FAQs
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FAQs
Browse our frequently asked technical questions by selecting a product below.
What is the error rate of UltraScript™ Reverse Transcriptase?
Like the great majority of the RTases available on the market UltraScript™ Reverse Transcriptase, as well as all other RTases we offer, are derived from wild-type Moloney Murine Leukemia Virus (MMLV) RTase and have an error rate of 1×10-4 errors/bp1. This also applies to RTases derived from Avian Myeloblastosis Virus (AMV).
Running the reaction at a higher temperature increases fidelity because it destabilises mismatched base pairs2. If low fidelity is required adding manganese will make RTases highly mutagenic and could increase their speed3.
1 Yasukawa, K. et al. Next-generation sequencing-based analysis of reverse transcriptase fidelity. Biochem Biophys Res Commun 492, 147-153, doi:10.1016/j.bbrc.2017.07.169 (2017).
2 Malboeuf, C. M., Isaacs, S. J., Tran, N. H. & Kim, B. Thermal effects on reverse transcription: improvement of accuracy and processivity in cDNA synthesis. Biotechniques 30, 1074-1078, 1080, 1082, passim, doi:10.2144/01305rr06 (2001).
3 Cadwell, R. C. & Joyce, G. F. Randomization of genes by PCR mutagenesis. PCR Methods Appl 2, 28-33 (1992).
