FAQs
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FAQs
Browse our frequently asked technical questions by selecting a product below.
Can UltraScript 2.0 Reverse Transcriptase do template switching?
All our RTases can be used in template switching applications. We currently do not provide a full protocol or dedicated kit but we recommend referring to this list of articles for reference1-4.
1 Takada, S. & Mano, H. Profiling of microRNA expression by mRAP. Nat Protoc 2, 3136-3145, doi:10.1038/nprot.2007.457 (2007).
2 Picelli, S. et al. Full-length RNA-seq from single cells using Smart-seq2. Nat Protoc 9, 171-181, doi:10.1038/nprot.2014.006 (2014).
3 Moldovan, N. et al. Multi-Platform Sequencing Approach Reveals a Novel Transcriptome Profile in Pseudorabies Virus. Front Microbiol 8, 2708, doi:10.3389/fmicb.2017.02708 (2017).
4 Zajac, P., Islam, S., Hochgerner, H., Lonnerberg, P. & Linnarsson, S. Base preferences in non-templated nucleotide incorporation by MMLV-derived reverse transcriptases. PLoS One 8, e85270, doi:10.1371/journal.pone.0085270 (2013).
