FAQs

No. UNG is more temperature-sensitive than DNA polymerases and should be kept cool (on ice or cooling blocks) during reaction setup. The 20x UNG Additive should also be kept cold at all times with handling limited to the minimum necessary for thawing and reaction setup. An additional UNG activity step is also included in the qPCR thermal cycling program for UNG kits, to allow this enzyme to eliminate any previously amplified uracil-containing DNA.

Neither the blue dye or SampleTrack Buffer, when used at the recommended concentration, inhibit PCR/qPCR reactions or quench qPCRBIO SyGreen® fluorescence. No change to cycling conditions is required when using SampleTrack Buffer as recommended as part of this kit.

The qPCRBIO SyGreen® Blue Mix provided as part of this kit is blue, and SampleTrack Buffer is yellow. When a sample containing SampleTrack Buffer is added to the blue mastermix, the reaction turns green, providing an immediate visual indication that sample addition has occurred.

No. The qPCRBIO SyGreen® Chameleon Kit is a dye-based qPCR product. For probe-based qPCR, use appropriate probe-based ranges such as Clara® Probe Mixes or Clara® Probe 1-Step Mixes. These can be combined with standalone SampleTrack Buffer for similar colour-change benefits.

The main benefit is visual setup confirmation. The kit helps users track both mastermix and sample addition during qPCR setup, reducing the risk of failed reactions caused by omitted reagents or missed samples.

The kit is designed for dye-based qPCR using qPPCRBIO SyGreen® detection. It is suitable for applications such as relative gene expression, absolute quantification and 2-step RT-qPCR workflows.