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PCR Application Notes

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Find application notes and product bulletins on a range of PCR-based methods.

Welcome to our comprehensive collection of PCR application notes and product bulletins, designed to inspire and guide your research. Here, you’ll find a diverse range of applications, from pathogen detection to metagenomic NGS analysis, each showcasing the versatility and precision of our PCR products. Dive into these notes to discover how our technologies can help in your experiments, providing you with the tools for groundbreaking discoveries in molecular biology.

Air-Dryable Probe Mixes

Discover a breakthrough in diagnostics with air-dryable qPCR reagents, allowing in-house drying in under 90 minutes. This technology eliminates cold-chain needs, offers stability at room temperature, and maintains sensitivity for virus detection, revolutionizing point-of-care testing with cost-effective, humidity-resilient assays.

Air-Dryable Inhibitor-Tolerant Probe Mixes

Learn about the benefits of utilising Air-Dryable Inhibitor-Tolerant Mixes to revolutionize your qPCR diagnostics. These mixes allow for the in-house creation of stable, room-temperature tests that resist common inhibitors found in samples like saliva and blood. Simply dry the mix in under 90 minutes, store at ambient conditions for up to 12 months, and achieve sensitive detection even with challenging samples. This approach simplifies diagnostics, making accurate testing more accessible and cost-effective.

Clara® Probe Mix

Designed to support efforts in quickly diagnosing the disease, this application note can also be used as a guide to set up multiplex assays for the detection of any DNA virus or target.

Clara® Probe and Probe 1-Step Mixes AquaPlex

This application note demonstrates how substituting Cy5 with Texas Red in Clara® AquaPlex Mixes enhances the sensitivity and performance of multiplex qPCR and 1-step RT-qPCR assays, making them ideal for applications where accurate detection and reliable results are critical.

Clara® HRM Mix

A brief demonstration of how a good quality HRM mix can be used to quantify methylation in specific target sequences. Ideal for epigenetic analysis workflows.

qPCRBIO Probe 1-Step Virus Detect

Reliable pathogen and variant detection is key to both prevention and therapy of infectious disease, and RT-qPCR is an ideal method to do so. However, optimising a multiplex assay takes time and effort. That’s why we’ve written this application note describing a rapid and sensitive protocol to detect SARS-CoV-2 and three common winter viruses, so you can go straight to testing samples.

Discover an efficient method for detecting SARS-CoV-2 using multiplex RT-qPCR, optimized for rapid, sensitive, and high-throughput diagnostic testing. This approach enhances COVID-19 management by enabling early detection and differentiation from other respiratory viruses.

VeriFi® Library Amplification Mix

Improve metagenomic analysis using VeriFi® Library Amplification Mix. Discover the benefits of more unique reads and reduced GC-bias in NGS.

Learn about how VeriFi® Library Amplification Mix and VeriFi® Hot Start Polymerase tolerate a range of blood, laboratory chemical, and environmental inhibitors.