FAQs

In the product manual we are providing a general lyophilisation protocol, tested for 2 mL glass vials filled with 500 μL of the Lyo-Ready Probe 1-Step Mix. We observed that lyophilisation is facilitated (i.e., the program is shorter, and cakes have a more uniform aspect with no signs of shrinkage) when the Lyo-Ready Probe 1-Step Mix is diluted to 1x or 2x (with oligonucleotides and water), so we recommend doing this dilution before starting the run.

Depending on the volume lyophilised, the material of the vials (i.e., glass vs plastic) and the lyophiliser used, the cycle may be optimised.

If the cakes are not properly formed after the cycle, we recommend repeating with a more conservative cycle, in which the primary drying is carried at -50 °C and time is extended to guarantee the correct cake formation. Secondary drying phase could also be extended if required.