FAQs

Both products are derived from wild-type MMLV reverse transcriptase and contain multiple mutations that enhance their function. They are each excellent RTases in their own right. UltraScript™ 2.0 is more thermostable up to 65 °C and above, resistant to inhibitors and capable of reverse transcribing large quantities of RNA up to 3.5 μg. UltraScript™ 2.0 can be also used for applications requiring sensitivity due to a low amount of RNA present, however, this requires substantial optimisation on the users side using the current formulation and we recommend using UltraScript™ instead. UltraScript™ can be used with 1-step kits whereas UltraScript™ 2.0 should be used only as a part of the 2-step process (RT then PCR). UltraScript™ 2.0 is especially recommended for long and difficult templates that contain large amounts secondary structure. Both RTases can be used with cDNA synthesis kit with/or without hexamers and oligo-d(T).