FAQs

Both products are derived from wild-type MMLV reverse transcriptase and contain multiple mutations that enhance their function. They are each excellent RTases in their own right. UltraScript 2.0 is more thermostable up to 65°C and above, resistant to inhibitors and capable of reverse transcribing large quantities of RNA up to 3.5 μg. UltraScript 2.0 can be also used for applications requiring sensitivity due to a low amount of RNA present, however, this requires substantial optimisation on the users side using the current formulation and we recommend using UltraScript instead. UltraScript can be used with 1-step kits whereas UltraScript 2.0 should be used only as a part of the 2-step process (RT then PCR). UltraScript 2.0 is especially recommended for long and difficult templates that contain large amounts secondary structure. Both RTases can be used with cDNA synthesis kit with/or without hexamers and oligo-d(T).