FAQs

UltraScript 2.0 has an excellent ability to handle various RT inhibitors including those found in blood and plasma. We recommend optimisation by performing a titration of the amount blood/plasma sample for RT reaction, varying the amount enzyme and diluting cDNA product. Refer to “How can high Ct’s in the qPCR step or low PCR product on the gel after a RT step (PCR/qPCR problem) be alleviated?” for further fine tuning of the protocol.