FAQs
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FAQs
Browse our frequently asked technical questions by selecting a product below.
Why is there an earlier Ct when using qPCRBIO SyGreen Mixes compared to a competitor’s product?
An early Ct could indicate one of two things. The first could be the result of our product performing very well relative to the competitor’s product. We have designed our products to be faster than our competitors, and we have found that we are significantly faster than many.
The second could be that caused by non-specific products. This can be verified by running a melt analysis at the end of the qPCR run. If your results show one peak, it is likely the correct product was amplified. You may observe the position of the peak being different with our mix relative to our competitor’s mix but is this normal because the salt concentration and pH affect the melting temperature. If your results show more than one peak, then the reaction is not specific. PCR products can also be run on an agarose gel to verify if there is only one band, and if it is the same size as the amplicon obtained with the competitor’s product.
To address the issue of a non-specific product, the thermal cycling will need to be adjusted.
