FAQs
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FAQs
Browse our frequently asked technical questions by selecting a product below.
What are the ROX concentrations in qPCRBIO SyGreen Mixes?
The qPCRBIO SyGreen Mixes that contain passive reference dyes come in different formulations, each with a different concentration of the passive reference dye:
- qPCRBIO SyGreen Mix Lo-ROX (PB20.11) contains 112 nM ROX.
- qPCRBIO SyGreen Mix Hi-ROX (PB20.12) contains 1.12 µM ROX.
- qPCRBIO SyGreen Mix Fluorescein (PB20.13) contains 0.2 mM fluorescein.
- qPCRBIO SyGreen Mix Separate-ROX (PB20.14) 2x mix contains no ROX and include a separate tube of 50 µM ROX additive. This enables you to choose what concentration of ROX you’d like to use.
You can use our qPCR Selection Tool under the Resources drop-down menu to determine which of our mixes are best suited for your qPCR machine.
