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FAQs
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qPCRBIO SyGreen Mixes contain the dye SyGreen. Does it belong to the SYBR green family?
SyGreen belongs to the family of asymmetric cyanine dyes, which is the same family that SYBR™ Green belongs to. SyGreen is an improved, new generation dye from this family. Similar to SYBR™ Green, SyGreen intercalates the DNA. Unlike SYBR™ Green, SyGreen supports PCR amplification over a wide range of dye concentrations, that is, the dye does not inhibit the PCR reaction. This plays a role in producing robust and consistent DNA melting curves both with prokaryote and eukaryote templates, as it shows no preference in nucleotide composition.
