FAQs
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FAQs
Browse our frequently asked technical questions by selecting a product below.
What troubleshooting is recommended if there are non-specific products using qPCRBIO Probe Mixes?
There are different options to consider when optimising the reaction:
- Reduce the annealing/extension time to 5 seconds
- Increase the annealing/extension temperature from 60 to 65°C
- Dilute the DNA template by starting with 5ng of DNA and using a 10x template dilution series. In addition to running these on a gel to see if the non-specific products persist, the efficiency of the reaction can be calculated with the software of the qPCR instrument after doing the template dilution. If the efficiency is between 90 – 110%, then the amplicon is being doubled every cycle.
