FAQs
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FAQs
Browse our frequently asked technical questions by selecting a product below.
What should be considered if the normalisation of signal with ROX appears to be lower relative to a competitor’s mix?
Ensure you are using the right concentration of ROX because different instruments require different ROX concentrations. For example, if a qPCRBIO Probe Mix Hi-ROX is used with an instrument requiring a Lo-ROX mix, the software will normalise the qPCRBIO signal against the Hi-ROX level. This will significantly reduce the fluorescence level of the qPCRBIO mix relative to competitors’ mixes, if in that case the Lo-ROX mix was chosen for the competitor.
When comparing mixes from different manufacturers, it is better to carry out separate runs or turn the passive reference off before analysing data.
