FAQs
Get the most out of your enzyme with our documents, guides, brochures and tools
FAQs
Browse our frequently asked technical questions by selecting a product below.
What troubleshooting is recommended when Ct values are higher than usual?
Higher Ct values are generally indicative of delayed amplification. This could most likely be due to an excess of template in the reaction resulting in the primers and probes being bound on different DNA molecules. Samples usually have a lot of DNA other than the target gene and this can scatter the oligos. We recommend diluting the samples (10x-1000x) to solve this.
Moreover, annealing/extension temperature can also be increased to make the binding of the oligos more specific to the target sequence and decrease background signal.
