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FAQs

Browse our frequently asked technical questions by selecting a product below.

What are the differences between probe and dye-based mixes?

Probe based kits such as qPCRBIO Probe 1-Step Virus Detect offer higher sensitivity and are unlikely to show non-template amplifications. Multiplexes can be measured using amplicons with different fluorophores for specific probes, which cannot be achieved using dyes.

Dye-based systems such as qPCRBIO SyGreen 1-Step Detect and 1-Step Go detect any intact dsDNA and will therefore show primer dimers and off-target/non-template amplifications. These can be separated from product peaks by analysing melting curves.

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