FAQs
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FAQs
Browse our frequently asked technical questions by selecting a product below.
What should the length of the amplicon be?
For efficient amplification under fast cycling conditions we recommend amplicon lengths between 80bp and 200bp. With all manufacturers master mixes the shorter the amplicon length the faster the reaction can be cycled. Amplicon lengths should not exceed 400bp. Primers should have a predicted melting temperature of around 60°C, using default Primer 3 settings (http://frodo.wi.mit.edu/primer3/).
