FAQs
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FAQs
Browse our frequently asked technical questions by selecting a product below.
What is the recommended extension time for VeriFi™ Polymerase?
30 seconds per kilobase (kb) is recommended for amplification from eukaryotic DNA. Shorter extensions times are possible provided there isn’t too much template DNA being loaded. If there are non-specific bands present after amplification the amount of template DNA should be decreased. 2-step protocols which combine annealing and extension are also possible. Optimisation of the cycling conditions and template concentration may be required if you deviate from the conditions and concentration recommended in the manual.
