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Browse our frequently asked technical questions by selecting a product below.
Can I use PCRBIO Ultra Polymerase if my assay requires a specialized buffer?
The 5x PCRBIO Ultra Buffer supplied with PCRBIO Ultra Polymerase has been developed specifically for this enzyme and we highly recommend using them together. However, PCRBIO Ultra Polymerase should be compatible with any PCR buffer developed for use with wild-type Taq. If you use a customised buffer with PCRBIO Ultra Polymerase, keep in mind reaction parameters such as annealing temperature and concentrations of the enzyme, template, dNTPs and MgCl2, may require optimisation.