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Browse our frequently asked technical questions by selecting a product below.
Would the PCR reaction work if my sample has a high level of inhibitors (like in blood and stool samples)?
PCRBIO Rapid Extract PCR Kit can be used with human blood however, blood components may inhibit the PCR reaction. Therefore, it’s a good idea to perform a serial dilution of the extracted DNA in order to find the optimal template concentration for the PCR amplification.
If the amplification is less than optimal, we recommend performing an isopropanol/acetate precipitation of the DNA prior to the PCR reaction.