Get the most out of your enzyme with our documents, guides, brochures and tools
Browse our frequently asked technical questions by selecting a product below.
Which samples can be used with PCRBIO Rapid Extract PCR Kit?
Rapid Extract PCR Kit was tested with the following list of tissues
- Mammalian cell cultures
- Animal tissues from a variety of organisms (Fish, lobster, Mice tails / ears, Zebrafish, Nematodes, Kidney, Hair Follicle)
- Insects like Drosophila, mosquitos, beetle (legs, wings)
- Buccal swabs
- Gram positive bacteria
- Fungal Mycelia
- Stool samples
- Fish Scale clips
The following tissues can require a pre-wash step before starting the PCRBIO Rapid Extract protocol2
- Yeast2,3 (pre-wash or digestion with zymolase to obtain spheroblasts)
- Feather4 (requires modification of the protocol see below )
- Stool sample5 (requires dilution series to get rid of inhibitors)
If you have a small amount of starting material, we recommend keeping the extracted DNA in the lowest volume possible and to avoid any further dilutions after inactivating the protease.
1 Batubara, A. et al. 2016. Study of BMP15 gene polymorphism in Boer, Kacang, and Boerka goats. Jurnal Ilmu Ternak Dan Veteriner 21(4): 224-230. DOI: http://dx.doi.org/10.14334/jitv.v21i4.1636 (2016).
2 Inglis, P.W. et al. 2018. Fast and inexpensive protocols for consistent extraction of high quality DNA and RNA from challenging plant and fungal samples for high throughput SNP genotyping and sequencing Applications. PLOS ONE | https://doi.org/10.1371/journal.pone.0206085 October 18.
3 Suzuki, T. & Iwahashi, Y. 2013. RNA preparation of Saccharomyces cerevisiae using the digestion method may give misleading results. Appl Biochem Biotechnol 169:1620–1632
4 Bello, N. et al. 2001. Isolation of genomic DNA from feathers. J Vet Diagn Invest 13:162–164
5 Nantavisai, K. et al. 2007. Evaluation of the sensitivities of DNA extraction and PCR methods for detection of Giardia duodenalisin stool specimens. J. Clinical Microbiology. 45: 581–583 doi:10.1128/JCM.01823-06