FAQs

Yes. Here is an easy protocol for DNA extraction from nematodes.

1. Prepare the extraction solution by combining 2 μL 5x Rapid Extract Buffer A with 1 μL Rapid Extract Buffer B and 7 μL PCR-grade water (Cat. no. PB40.40).
2. Add 1 nematode (these volumes can also work for multiple nematodes per prep).
3. Incubate the samples for 5 min at 75 °C for sample lysis.
4. Deactivate the reation by incubating for 10 min at 95 °C.
5. At 90 μL to the deactivated extraction mix and centrifuge at maximum speed (~13,500 g) in a microcentrifuge for 1 min.
6. The supernatant can be used as template in PCR and qPCR immediately, or stored at -20 °C for later use.
7. Generally, 1-2 μL of supernatant is sufficient as template in a 20 μL PCR reaction.