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Can I use PCRBIO Rapid Extract PCR Kit to extract and amplify DNA from a few dozen cells on filter paper?
Our PCR mixes work reliably with 10 or more copies of template per reaction but not lower than that. Keep the extracted DNA in the lowest volume possible and don’t dilute further after inactivating the protease. We advise setting up the assay with more cells and once the conditions are established, scaling it down from there.