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FAQs

Browse our frequently asked technical questions by selecting a product below.

What is the difference between PCRBIO Taq DNA Polymerase and PCRBIO HS Taq DNA Polymerase?

PCRBIO HS Taq DNA Polymerase uses proprietary antibody-mediated hot start technology whereas PCRBIO Taq DNA Polymerase does not have this feature. The interaction of the antibody with the Taq DNA Polymerase leaves the enzyme inactive until the hot start step.

The hot start refers to the initial activation step at 95°C, which subsequently deactivates the antibody bound to the DNA polymerase. Inactivation of the Taq DNA Polymerase below 65°C prevents primer-dimer formation and non-specific amplification. This allows for specific amplification from low copy number target sequences.

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