FAQs
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FAQs
Browse our frequently asked technical questions by selecting a product below.
Can I use PCRBIO HiFi Polymerase if my assay requires a specialized buffer?
The 5x PCRBIO HiFi buffer supplied with PCRBIO HiFi Polymerase has been developed specifically for this enzyme and we highly recommend using them together. However, PCRBIO HiFi Polymerase should be compatible with any PCR buffer developed for use with Pfu polymerase. If you use a customised buffer with PCRBIO HiFi Polymerase, keep in mind reaction parameters such as annealing temperature and concentrations of the enzyme, template, dNTPs and MgCl2, may require optimisation.
