FAQs
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FAQs
Browse our frequently asked technical questions by selecting a product below.
My results show a very low yield. What trouble-shooting suggestions do you have?
You may want to consider the suggestions below and also refer to the literature1.
- Optimise the annealing temperature in an annealing temperature gradient PCR.
- Increase the amount of template in the reaction.
- Increase the number of cycles.
- Increase the amount of enzyme per reaction.
- Increase the primer concentration, but do not exceed 1 µM of each primer.
- Try a fresh dNTP solution.
- Optimise the MgCl2
1 Lorenz, T. C. Polymerase chain reaction: basic protocol plus troubleshooting and optimization strategies. J Vis Exp, e3998, doi:10.3791/3998 (2012).