FAQs
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FAQs
Browse our frequently asked technical questions by selecting a product below.
Can PCRBIO Classic Taq be used for colony PCR?
Yes. If you’re working from bacterial colonies use a sterile tip to pick a colony and re-suspend into the 50µl PCR reaction. If working from liquid culture add 5µl of overnight culture to the final mix. Follow the general protocol and increase the initial denaturation time to 10 min at 95°C.
